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Isolation and characterization of polymorphic microsatellite markers from Australia short-finned eel (Anguilla
Xiao-Ling Gong1, Si-Fa Li, Wan-Qi Cai
1Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Fisheries University, Ministry of Education, No. 334 Jungong Road, Shanghai 200090, China.
Abstract:
Seventeen microsatellite DNA loci from the Australian short-finned eel (Anguilla australis Richardson) were isolated and their amplification characteristics were described. The polymerase chain reaction primers were tested on 40 eel individuals. The primers amplified loci with relatively high numbers of alleles, ranging from five to 14 with an average of nine per locus. Mean observed heterozygosity (H(O) ) and expected heterozygosity (H(E) ) were 0.6779 and 0.7374, respectively, indicating that these markers would be useful for population studies. No loci deviated significantly from Hardy-Weinberg equilibrium (P = 0.05) and no evidence was found for genotypic disequilibrium among loci at a 5% significance level.
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