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Published on: January 6, 2015
Microsatellites from kousa dogwood (Cornus kousa)
Phillip A Wadl1, Xinwang Wang, Brian E Scheffler
1Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS-CGRU MSA Genomics Laboratory, 141 Experiment Station Road, Stoneville, MS 38776, USA, USDA-ARS Southern Horticultural Laboratory, 810 Highway 26 West, Poplarville, MS 39470, USA.
Abstract:
Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ranged from 0 to 0.3 and expected heterozygosity ranged from 0.38 to 0.91. These microsatellites will be useful in population studies, and a breeding programme for cultivar development of Cornus species.
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