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Published on: January 6, 2015
Microsatellites from kousa dogwood (Cornus kousa).
Phillip A Wadl1, Xinwang Wang, Brian E Scheffler
1Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS-CGRU MSA Genomics Laboratory, 141 Experiment Station Road, Stoneville, MS 38776, USA, USDA-ARS Southern Horticultural Laboratory, 810 Highway 26 West, Poplarville, MS 39470, USA.
Researchers identified microsatellite loci in Cornus kousa, developing eight optimized primer pairs. These markers are polymorphic and valuable for population studies and breeding programs in Cornus species.
Area of Science:
- Plant genetics
- Molecular biology
- Horticulture
Background:
- Microsatellites are powerful tools for genetic analysis.
- Cornus kousa cultivar development requires robust genetic markers.
Purpose of the Study:
- To identify and characterize microsatellite loci in Cornus kousa.
- To develop and optimize primer pairs for genetic analysis of Cornus kousa cultivars.
Main Methods:
- Microsatellite loci identification in Cornus kousa.
- Primer pair development for 86 loci.
- Optimization and screening of eight primer pairs using genomic DNA from 22 cultivars.
Main Results:
- All eight optimized loci were polymorphic.
- The number of alleles per locus ranged from 3 to 17.
- Observed heterozygosity ranged from 0 to 0.3, and expected heterozygosity ranged from 0.38 to 0.91.
Conclusions:
- Developed microsatellite markers are effective for Cornus kousa.
- These markers will aid population studies and cultivar development programs for Cornus species.
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