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Updated: Jun 1, 2026
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Eleven novel microsatellite markers for the Asian oyster Crassostrea ariakensis
Jie Xiao1, Jan F Cordes, Kenneth C Jones
1VIMS, The College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, USA, Genetic Identification Service, 9552 Topanga Canyon Blvd., Chatsworth, CA 91311, USA.
Abstract:
Clones from four partial genomic libraries of the Asian oyster Crassostrea ariakensis were screened to reveal 64 microsatellite-containing sequences. Polymerase chain reaction primers were designed for 34 of these loci. Reactions were optimized for 11 primer pairs and markers were evaluated in eight family crosses and one wild population from northern China. Zero to three null alleles per locus were identified in family crosses, and two of the 11 loci showed allele frequency deviations from Hardy-Weinberg equilibrium in the wild population. These markers have proven useful for genetic studies of C. ariakensis.
