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Updated: Jun 1, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation and characterization of polymorphic microsatellite and COI loci from the whelk Kelletia kelletii
1Department of Ecology, Evolution & Marine Biology, University of California, Santa Barbara, CA 93106, USA, Hawaii Institute of Marine Biology, University of Hawaii at Manoa, Kaneohe, HI 96744, USA.
Abstract:
There is considerable interest in the genetic structure of Kelletia kelletii because of its economic, ecological and scientific importance. To that end, we developed species-specific primers which amplify mitochondrial cytochrome c oxidase subunit I as well as 13 hypervariable nuclear microsatellite loci. Using dye-labelled primers, the microsatellite loci can be co-amplified in two multiplex polymerase chain reactions and scored simultaneously on an automated sequencer.
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