Development of highly polymorphic tetranucleotide microsatellite markers in Austrocedrus chilensis
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Published on: August 12, 2009
María Verónica Arana1, Anna Buonamici, Federico Sebastiani
1Unidad de Genética Forestal, Instituto Nacional de Tecnología Agropecuaria, EEA Bariloche, CC277, 8400 Bariloche, Argentina, Istituto di Genetica Vegetale, CNR, 50019 Sesto Fiorentino (Firenze), Italy, Dipartimento di Biotecnologie Agrarie, Laboratorio Genexpress, 50019 Sesto Fiorentino (Firenze), Italy, Departamento Sistemas y Recursos Forestales, CIFOR-INIA, 28040 Madrid, Spain.
Abstract:
An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Numbers of alleles ranged from 3 to 19 and levels of observed heterozygosity among the 9 loci ranged from 0.32 to 0.95. No locus combinations exhibited linkage disequilibrium. These polymorphic markers will be useful tools for the study of demography and gene flow and more in general for population and conservation genetics of this species.
