Related Experiment Video
Updated: Jun 1, 2026

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Published on: August 2, 2011
Isolation of 10 tetranucleotide microsatellite loci in the blackcap (Sylvia atricapilla)
Gernot Segelbacher1, Gregor Rolshausen, Tanja Weis-Dootz
1Department of Wildlife Ecology and Management, University Freiburg, Tennenbacher Str. 4, D-79106 Freiburg, Germany, Department of Evolutionary Biology and Animal Ecology, Faculty of Biology, University Freiburg, Hauptstrasse 1, D-79108 Freiburg, Germany, Estación Biológica de Doñana (CSIC), Pabellón del Perú, Avda. Maria Luisa s/n, 41013 Sevilla, Spain.
Abstract:
We isolated 10 polymorphic microsatellite DNA loci from the blackcap (Sylvia atricapilla) and optimized them for future studies of population differentiation in populations with different migration strategies in southwestern Germany. The loci were screened for polymorphism using 178 individuals from two populations in Germany and Spain. The primers amplified highly variable loci characterized by two to 19 alleles per locus and their observed and expected heterozygosities range from 0.47 to 0.81 and from 0.50 to 0.91, respectively.

