Related Experiment Video
Updated: Jun 1, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Polymorphic microsatellite markers for the Mojave desert tortoise, Gopherus agassizii
B E Hagerty1, M M Peacock, V S Kirchoff
1Program in Ecology, Evolution, and Conservation Biology, Department of Biology, MS/314, University of Nevada, Reno, Reno, NV 89557, USA.
New microsatellite markers were developed for the Mojave desert tortoise (Gopherus agassizii). These genetic markers will aid conservation efforts by improving the efficiency of population and genetic diversity studies for this threatened species.
Area of Science:
- Genetics
- Conservation Biology
- Wildlife Management
Background:
- The Mojave desert tortoise (Gopherus agassizii) is a threatened species facing significant conservation challenges.
- Effective conservation strategies require robust genetic data to understand population structure and diversity.
Purpose of the Study:
- To develop and characterize novel microsatellite markers for the Mojave desert tortoise.
- To provide tools for enhanced genetic analysis in conservation programs for this species.
Main Methods:
- Development of 14 tri- and tetranucleotide microsatellite loci.
- Polymerase chain reaction (PCR) conditions were optimized for amplification.
- Analysis of genetic variation across three populations (87 individuals) in the Mojave Desert.
Main Results:
- Markers exhibited a high number of alleles per locus (4-33) and substantial observed heterozygosity (average 0.733).
- No significant linkage disequilibrium was detected between loci.
- No consistent deviation from Hardy-Weinberg equilibrium was observed, indicating marker reliability.
Conclusions:
- The developed microsatellite markers are effective for population genetics studies of Gopherus agassizii.
- These markers are suitable for PCR multiplexing, enabling high-throughput genetic analysis.
- The tools will significantly support conservation genetics research and management of the threatened Mojave desert tortoise.
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
DNA Microarrays
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

