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New microsatellite markers isolated from mungbean (Vigna radiata (L.) Wilczek)
Prakit Somta1, Warunee Musch, Buppa Kongsamai
1Department of Agronomy, Faculty of Agriculture, DNA Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Rice Gene Discovery Unit, National Center for Genetic Engineering and Biotechnology, Asian Regional Center-AVRDC-The World Vegetable Center, Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom 73140, Thailand Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
Abstract:
In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heterozygosity varied from 0 to 0.133, while expected heterozygosity ranged from 0.095 to 0.498. Tests for Hardy-Weinberg equilibrium (HWE) and pairwise linkage disequilibrium of the polymorphic loci revealed that all loci except MB-SSR14 significantly departed from HWE and four pairwise combinations, viz. MB-SSR14 vs. MB-SSR42, MB-SSR42 vs. MB-SSR87, MB-SSR114 vs. MB-SSR121, and MB-SSR175 vs. MB-SSR231 significantly deviated from linkage disequilibrium. The markers are being used to study genetic diversity and genome mapping of mungbean.
