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Related Experiment Video

Updated: Jun 1, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

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Published on: July 11, 2025

DNA barcoding discriminates echinoderm species.

Robert D Ward¹, Bronwyn H Holmes, Tim D O'Hara

¹CSIRO Wealth from Oceans Flagship, CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart, Tas. 7001, Australia, Museum Victoria, PO Box 666, Melbourne, Vic. 3001, Australia.

Molecular Ecology Resources

|May 19, 2011

Summary

This summary is machine-generated.

DNA barcoding using mitochondrial cytochrome oxidase I (COI) sequences effectively distinguishes most Echinodermata species. This method aids in identifying species across all five echinoderm classes, with potential for detecting cryptic speciation.

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Area of Science:

Marine Biology
Genomics
Taxonomy

Background:

Echinodermata, encompassing five classes (Ophiuroidea, Asteroidea, Echinoidea, Holothuroidea, Crinoidea), presents taxonomic challenges.
DNA barcoding, utilizing specific gene sequences, offers a potential tool for species identification and differentiation.

Purpose of the Study:

To assess the efficacy of DNA barcoding, specifically the mitochondrial cytochrome oxidase I (COI) gene, for species diagnosis across all five classes of Echinodermata.
To evaluate intraspecific and congeneric sequence divergence within Echinodermata using COI barcodes.

Main Methods:

DNA barcode sequences (657-bp COI gene segment) were collected from 503 specimens representing 191 species of Echinodermata.
Sequences were sourced from GenBank and newly generated for the study.
Phylogenetic analyses, including neighbor-joining trees, were constructed to assess relationships and divergences.

Main Results:

A high success rate of 97.9% (187 out of 191 species) was achieved in distinguishing species using COI barcodes.
Low mean intraspecific divergence (0.62%) and higher mean congeneric divergence (15.33%) were observed.
Potential instances of cryptic speciation were identified, and unusual phylogenetic placements were noted for two ophiuran species.

Conclusions:

DNA barcoding of the COI gene is a highly effective and accurate method for species-level identification within Echinodermata.
The approach demonstrates utility across all five major echinoderm classes, aiding taxonomic resolution and potentially revealing cryptic biodiversity.