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Development of simple sequence repeat markers for the soybean rust fungus, Phakopsora pachyrhizi
Sharon J Anderson1, Christine L Stone, Martha Lucia Posada-Buitrago
1Foreign Disease-Weed Science Research Unit, Agricultural Research Service, US Department of Agriculture, Fort Detrick, Frederick, MD 21702, USA, Joint Genome Institute and Lawrence Berkeley National Laboratory, US Department of Energy, Walnut Creek, CA 94598, USA, Advanced Technology Program, Advanced Biomedical Computing Center, SAIC-Frederick Inc., NCI-Frederick, Frederick, MD 21702, USA.
Abstract:
Twenty-four simple sequence repeat markers were developed for Phakopsora pachyrhizi, a fungal pathogen of soybean (Glycine max) and other legumes. All 24 of the loci were evaluated on 28 isolates of P. pachyrhizi. Twenty-one loci were polymorphic, with allelic diversity ranging from two to eight alleles, and null alleles were observed for eight of the 24 loci. A preliminary screen with the closely related species, P. meibomiae, indicated that these primer pairs are specific to P. pachyrhizi.
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