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Published on: October 5, 2017
Development of nine microsatellite markers for Pomacentrus amboinensis
David B Jones1, Dean R Jerry, Mark I McCormick
1School of Marine and Tropical Biology, James Cook University, Townsville, Qld 4811, Australia, ARC Centre of Excellence for Coral Reef Studies, Townsville, Qld 4811, Australia.
Abstract:
The relatively long pelagic larval duration of Pomacentrus amboinensis, a tropical fish, suggests the potential for long-distance dispersal; however, several nongenetic studies have found substantial self-recruitment at one location. To analyse patterns of connectivity of this species, primers for nine independent microsatellite loci were developed for P. amboinensis using a magnetic bead enrichment protocol. Twenty individuals from one location were analysed and observed heterozygosities ranged from 0.7 to 0.95. Eight of nine loci were in Hardy-Weinberg equilibrium and no evidence of linkage or null alleles were found.
