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Development of 10 tri- and tetranucleotide microsatellite loci for population studies in the common carp (Cyprinus
1Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China, College of Aqua-life Science and Technology, Dalian Fisheries University, Dalian 116023, China, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Abstract:
Ten tri- and tetranucleotide microsatellite DNA markers were isolated and characterized from common carp (Cyprinus carpio L.) to estimate genetic potential. These markers were tested in the samples from two closely related carp populations (Cyprinus carpio var. xingguonensis and Cyprinus carpio var. wananensis). The number of the alleles ranged from three to nine, and observed and expected hererozygosities varied from 0.207 to 1.000 and from 0.499 to 0.900 in each population, respectively. No evidence for linkage disequilibrium was found, indicating that these markers will be useful for population studies.
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