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Development of polymorphic microsatellite markers in Hessian fly, Mayetiola destructor (Say)
Brandon J Schemerhorn1, Yan M Crane, Philip K Morton
1USDA-ARS, Purdue University, 901 W. State Street, West Lafayette, IN 47907-2089, USA, Department of Entomology, Smith Hall, Purdue University, 901 W. State Street, West Lafayette, IN 47907-2089, USA.
Abstract:
A microsatellite library was prepared from size-selected genomic DNA of Hessian fly (Mayetiola destructor). Approximately 81% of recovered clones hybridized with microsatellite motif-specific probes. Subsequently, 2350 clones were sequenced. Sixty-two individual flies from laboratory strains were used to test for reliability and polymorphism in 50 of the microsatellites by gel electrophoresis; 18 were further tested with capillary electrophoresis. Of these, 17 behaved as a polymorphic single locus appropriate for population analysis.
