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Published on: March 13, 2011
Development and characterization of 11 microsatellite loci in a historically introduced carnivoran, the common genet
P Gaubert1, I Del Cerro, F Palomares
1Departamento de Biología de la Conservación, Departamento de Ecología Integrativa, Estación Biológica de Doñana, CSIC, Pabellón del Perú, Avda María Luisa s/n, 41013 Sevilla, Spain, UR IRD 131 - UMS MNHN 403, Département Milieux et Peuplements Aquatiques, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, France, Genética de la Conservación Animal, IRTA, Ctra de Cabrils, s/n 08348 Cabrils, Barcelona, Spain.
Abstract:
Microsatellite markers were developed to assess population structure and patterns of translocation in the introduced European common genet (Genetta genetta). Primer pairs were designed for 60 microsatellite sequences enriched for CA, GA, CATC and TAGA repeat motifs. Eleven loci that proved to be polymorphic were genotyped in 33 individuals from southwestern France. The number of alleles per locus and observed heterozygosities varied from three to seven and from 0.2121 to 0.7576, respectively. One locus (B103) showed significant departure from Hardy-Weinberg equilibrium, probably due to the presence of null alleles. Tests of linkage disequilibrium did not detect significant associations among loci.
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