Related Experiment Video
Updated: Jun 1, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Development of 11 polymorphic microsatellite loci in the small abalone (Haliotis diversicolor Reeve)
Peng Ren1, Zhiyong Wang, Cuiluan Yao
1Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China Department of Oceanography, Xiamen University, Xiamen 361005, China.
Abstract:
Eleven polymorphic microsatellite loci for Haliotis diversicolor were isolated and characterized from (CT)(n) - and (AC)(n) -enriched library. They were tested in 24 individuals from a natural population. All of them were polymorphic, with the number of alleles varying between three and 10. The observed heterozygosities and expected heterozygosities ranged from 0.083 to 0.913 and from 0.159 to 0.856, respectively. The 11 isolated microsatellite loci, except SA-JMU6 and SA-JMU12, followed Hardy-Weinberg expectations. Seven sets of primers also amplify in closely related species, H. ovina and H. asinine. These 11 polymorphic microsatellites will be useful for analysing the population structure and genetic diversity in H. diversicolor.
