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Development and characterization of novel, polymorphic microsatellite markers for oat crown rust, Puccinia coronata
Hattie R Dambroski1, Martin L Carson
1Cereal Disease Laboratory, Agricultural Research Service, US Department of Agriculture, 1551 Lindig Street, St Paul, MN 55108, USA.
Abstract:
We report the development of 37 novel and polymorphic microsatellite markers for oat crown rust, Puccinia coronata f.sp. avenae. The allelic diversity ranged from two to 16 alleles per locus. Observed heterozygosity ranged from 0.000 to 0.971, and expected heterozygosity from 0.057 to 0.848. Thirteen of the loci were not in Hardy-Weinberg equilibrium, due to either the presence of null alleles, small sample size, or the effects of population subdivision (Wahlund's effect). All 37 primer pairs were tested with P. graminis and P. triticina showing that they are specific to P. coronata.
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