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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Microsatellite markers isolated from a polyploid saltbush, Atriplex nummularia Lindl. (Chenopodiaceae)
Margaret Byrne1, Margaret Hankinson, Jane F Sampson
1Science Division, Department of Environment and Conservation, Locked Bag 104, Bentley Delivery Centre, Bentley, 6983, WA, Australia, CRC for Plant Based Management of Dryland Salinity, The University of Western Australia, Nedlands, 6009, WA, Australia.
Abstract:
Atriplex nummularia is a polyploid Australian saltbush which has been identified as a suitable species for use in the rehabilitation of agricultural land affected by salinity. We isolated 12 polymorphic loci for a preliminary assessment of genetic variability and structure within the species as a basis for a breeding programme. Preliminary screening of loci in 40 individuals from two populations revealed multibanded genotypes consisting of up to seven alleles in a single individual, with up to 29 alleles observed at a single locus. The multibanded patterns are consistent with the polyploid status of this species.
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