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Eight polymorphic microsatellite markers developed in the Chinese scorpion, Mesobuthus martensii (Scorpiones:
Ya-Jie Ji1, Liang Leng, Cheng-Min Shi
1State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China, Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
Abstract:
Eight polymorphic di- and trinucleotide microsatellite loci were developed in the Chinese scorpion, Mesobuthus martensii. The expected heterozygosity at these loci ranges from 0.019 to 0.860, with the observed allele numbers varying from two to 25. Overall, there were no deviations from Hardy-Weinberg equilibrium and no observed linkage disequilibrium after Bonferroni correction. Cross-species amplification of these loci in Mesobuthus eupeus revealed that five loci can amplify successfully in this species. The polymorphic microsatellite DNA markers reported here should provide helpful means to address questions concerning phylogeographical patterns and evolutionary history of M. martensii and closely related species.
