Polymorphic microsatellite DNA markers for Daviesia triflora (Papilionaceae)
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Published on: February 20, 2012
Tianhua He1, Siegfried L Krauss, Byron B Lamont
1Centre for Ecosystem Diversity and Dynamics, Department of Environmental Biology, Curtin University of Technology, PO Box U1987, Perth, WA 6845, Australia, Botanic Gardens and Parks Authority, Kings Park and Botanic Garden, West Perth, WA 6005, Australia, School of Plant Biology, The University of Western Australia, Nedlands, WA 6009, Australia.
Abstract:
We developed 11 polymorphic microsatellite DNA markers for an Australian native plant, Daviesia triflora. The number of alleles per locus in 40 individuals varied from four to 19, and observed and expected heterozygosities ranged from 0.450 to 0.925 and from 0.497 to 0.899, respectively. Nine loci showed no significant deviation from Hardy-Weinberg equilibrium (P > 0.05), and null alleles appear to exist at loci DT-A102 and DT-B103. All loci showed independent inheritance.
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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

