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Updated: Jun 1, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Twenty-three microsatellite DNA loci for population genetic studies and parentage assignment in orangethroat darter,
S P Hudman1, M J Grose, J B Landis
1Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA, Natural History Museum and Biodiversity Research Center, University of Kansas, Dyche Hall, 1345 Jayhawk Boulevard, Lawrence, KS 66045, USA, 1637 Merion Place, Lawrence, Kansas 66047, USA.
Abstract:
The genus Etheostoma is a species-rich and ecologically important group of fishes in North America. The orangethroat darter (Etheostoma spectabile) is widely distributed and abundant in headwater streams throughout the central Midwest, and is an excellent model for ecological and mating system studies. We developed 23 novel, polymorphic, and independent microsatellite loci for E. spectabile. We found from two to 14 alleles per locus, and observed heterozygosities ranged from 0.39 to 1.0. These markers, in combination with others isolated from Etheostoma taxa, will be useful for ecological and evolutionary studies in the genus.
