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Updated: Jun 1, 2026
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Development of simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus
Sukhuman Whankaew1, Sithichoke Tangphatsornruang, Kanokporn Triwitayakorn
1Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakhonpathom 73170, Thailand, National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand, Center for Cassava Molecular Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Abstract:
In this study, we describe the development of expressed sequence tag-simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus monodon) deposited in public sequence databases. A total of 46 primer pairs were designed and screened on 26 individuals of P. monodon from a natural population. Of these, 16 primer pairs showed polymorphic profiles with between two and five alleles per locus. The average unbiased and direct count heterozygosities were 0.4662 and 0.3516, respectively. Cross-amplification was tested with five individuals of Penaeus vannamei and polymorphic products were detected at five loci.
