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Updated: Jun 1, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Characterization of 18 new microsatellite loci in Atlantic cod (Gadus morhua L.)
Sigurlaug Skirnisdottir1, Christophe Pampoulie, Sigurbjorg Hauksdottir
1Matis-Prokaria, Gylfaflöt 5, 112 Reykjavik, Iceland, Marine Research Institute, Skulagata 4, 101 Reykjavik, Iceland, Department of Biology, University of Iceland, Sturlugata 7, 101 Reykjavik, Iceland.
Abstract:
Eighteen new microsatellite loci consisting of 10 di-, 5 tri-, 2 tetra- and 1 heptanucleotide repeats are introduced for the Atlantic cod (Gadus morhua L.). All loci were co-amplified in two polymerase chain reactions (plus two previously published microsatellites) and all products were typed clearly. The number of alleles per locus ranged from six (PGmo130) to 45 (PGmo76) and the observed heterozygosity ranged from 0.356 (PGmo130) to 0.957 (PGmo95). All loci except one followed Hardy-Weinberg expectations. Genetic linkage disequilibrium analysis between all pairs of loci did not yield any significant values.
