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Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell types have...

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Related Experiment Video

Updated: Jun 1, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

Using yeast as a model to study membrane proteins.

Julia Petschnigg1, Orson W Moe, Igor Stagljar

  • 1Department of Biochemistry, The Donnelly Centre, University of Toronto, Toronto, ON, Canada.

Current Opinion in Nephrology and Hypertension
|May 19, 2011
PubMed
Summary
This summary is machine-generated.

Yeast-based methods enable robust study of membrane protein-protein interactions (PPIs) in vivo. This research aids understanding of protein complexes for disease insights and drug discovery.

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

Related Experiment Videos

Last Updated: Jun 1, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cellular processes rely on stable or transient protein-protein interactions (PPIs).
  • Protein complexes function as molecular machines, with membrane proteins comprising a significant portion of the proteome and drug targets.
  • Understanding PPI networks is crucial for elucidating protein complex functions and identifying new drug targets.

Purpose of the Study:

  • To highlight the utility of yeast-based methods for studying membrane protein-protein interactions (PPIs).
  • To emphasize the importance of investigating PPIs for understanding cellular functions and disease mechanisms.
  • To explore the potential of PPI studies in defining new strategies for drug discovery.

Main Methods:

  • Utilizing yeast as a model organism to study membrane proteins, including transporters.
  • Employing techniques like split-ubiquitin membrane yeast two-hybrid and protein-fragment complementation assays.
  • Applying these methods for both large-scale genome-wide screens and smaller-scale PPI studies.

Main Results:

  • Yeast-based methods have proven successful and reliable for studying diverse membrane proteins.
  • These techniques are effective for both comprehensive genome-wide analyses and focused PPI investigations.
  • The application extends to various protein classes, demonstrating broad utility.

Conclusions:

  • Yeast-based in vivo methods provide a powerful platform for investigating membrane PPIs across different species, including mammals.
  • Mapping protein interactions serves as a foundation for studying poorly understood proteins and disease-related factors.
  • Identifying PPIs is vital for drug discovery, particularly for diseases linked to abnormal protein interactions.