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Related Experiment Video

Updated: Jun 1, 2026

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Published on: July 21, 2018

Ambient oxygen promotes tumorigenesis.

Ho Joong Sung¹, Wenzhe Ma, Matthew F Starost

¹Center for Molecular Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

|May 19, 2011

Summary

Reducing oxygen exposure significantly increased tumor-free survival in mice by decreasing DNA damage and RAG recombinase levels, highlighting oxygen's role in genomic instability and cancer development.

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Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance

Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance

Published on: February 14, 2025

Area of Science:

Oncology
Genetics
Molecular Biology

Background:

Oxygen is a known mutagen and causes genomic instability in mammalian cells.
The effect of ambient oxygen on de novo tumorigenesis at the organismal level remains unclear.

Purpose of the Study:

To investigate the impact of ambient oxygen levels on de novo tumorigenesis in vivo.
To determine if reducing oxygen exposure can inhibit cancer development.

Main Methods:

Experiments were conducted using p53-/- mice, with varying ambient oxygen exposure.
Levels of oxidative DNA damage and RAG recombinase were measured in the thymus.
Two additional cancer models (APC tumor suppressor gene and chemical carcinogenesis) were used to assess oxygen's role.

Main Results:

Decreasing ambient oxygen exposure increased median tumor-free survival time by approximately 50% in p53-/- mice.
Reduced oxygen levels led to decreased oxidative DNA damage and RAG recombinase in the thymus.
Oxygen was associated with genomic instability in APC and chemical carcinogenesis models.

Conclusions:

Ambient oxygen plays a critical role in promoting de novo tumorigenesis and increasing genomic instability in vivo.
Modulating oxygen exposure may represent a novel therapeutic strategy for cancer prevention.