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Immunohistochemical detection of nm23-H1/NDP kinase in childhood thyroid carcinoma
Y Shirahige1, J Irie, K Ashizawa
1NAGASAKI UNIV,SCH MED,DEPT INTERNAL MED 1,NAGASAKI 852,JAPAN. NAGASAKI UNIV,SCH MED,DEPT PATHOL 2,NAGASAKI 852,JAPAN. ITO HOSP,SHIBUYA KU,TOKYO 150,JAPAN.
Abstract:
Childhood thyroid cancer is known to be aggressive. High incidence of lymph node and distant metastasis are characteristic features of these cases. In adult, reduced expression of nm23-H1/nucleoside diphosphate (NDP) kinase has been correlated with cancer invasion and metastasis in some tumor types. Therefore, we examined the expression of nm23-H1 gene product in childhood thyroid carcinomas in Japan. 27 primary thyroid carcinomas and 8 metastatic lymph nodes were analyzed by immunohistochemistry using monoclonal antibody H1-229. 21 out of 23 cases (91%) of papillary carcinomas were positively immunostained, whereas none of the 4 follicular carcinomas showed any immunoreactivity. No correlation was found between the nm23-H1/NDP kinase antigen expression and nodal involvement or distant metastasis in primary tumors. However, only 50% (4 out of 8) of metastatic lymph nodes from papillary carcinoma were positively stained, demonstrating a significant decrease comparing to those of primary sites. These data indicate that the expression of nm23-H1/NDP kinase cannot predict tumor metastatic potential in childhood thyroid cancer.
Insights
nm23-H1/nucleoside diphosphate (NDP) kinase expression in childhood thyroid cancer does not predict metastasis. While present in most papillary tumors, its reduced presence in metastatic lymph nodes suggests nm23-H1/NDP kinase is not a reliable indicator of metastatic potential.
Area of Science:
- Oncology
- Molecular Biology
- Pathology
Background:
- Childhood thyroid cancer is aggressive with frequent metastasis.
- nm23-H1/nucleoside diphosphate (NDP) kinase is linked to invasion in adult cancers.
- Its role in pediatric thyroid cancer metastasis is unexplored.
Purpose of the Study:
- To investigate nm23-H1/NDP kinase expression in childhood thyroid carcinomas.
- To determine if nm23-H1/NDP kinase expression correlates with metastasis in pediatric thyroid cancer.
Main Methods:
- Immunohistochemistry was used to analyze nm23-H1/NDP kinase expression.
- 27 primary thyroid carcinomas and 8 metastatic lymph nodes were studied.
- Monoclonal antibody H1-229 was employed for detection.
Main Results:
- 91% of papillary thyroid carcinomas showed positive nm23-H1/NDP kinase staining.
- Follicular carcinomas exhibited no nm23-H1/NDP kinase immunoreactivity.
- No correlation was found between nm23-H1/NDP kinase expression and metastasis in primary tumors.
- A significant decrease in nm23-H1/NDP kinase staining was observed in metastatic lymph nodes compared to primary papillary tumors.
Conclusions:
- nm23-H1/NDP kinase expression does not predict metastatic potential in childhood thyroid cancer.
- The protein's role may differ between primary tumors and metastases.
- Further research is needed to understand nm23-H1/NDP kinase function in pediatric thyroid malignancies.
