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Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Related Experiment Video

Updated: Jun 1, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections
06:09

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Published on: February 9, 2020

Relationship between microlymphatic vessel density within tumors and lymph node metastasis.

Y Ohta1, M Tanaka, Y Endo

  • 1KANAZAWA UNIV,DEPT EXPT THERAPEUT,SCH MED,CANC RES INST,KANAZAWA,ISHIKAWA 920,JAPAN.

Oncology Reports
|May 19, 2011
PubMed
Summary

High microlymphatic vessel density (MLD) in tumors correlates with lymph node metastasis. This study in mice suggests MLD is a potential indicator for cancer spread, aiding in prognosis.

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Area of Science:

  • Oncology
  • Vascular Biology
  • Cancer Metastasis Research

Background:

  • Lymphatic vessel density (MLD) is implicated in tumor progression and metastasis.
  • Understanding the relationship between MLD and lymph node metastasis is crucial for cancer prognostication.

Purpose of the Study:

  • To investigate the correlation between microlymphatic vessel density (MLD) within tumors and the incidence of lymph node metastasis.
  • To evaluate MLD as a potential biomarker for predicting lymph node metastasis in specific human tumor xenografts.

Main Methods:

  • Utilized SCID mice xenograft models with various human tumor cells.
  • Employed enzyme-histochemistry for 5'-nucleotidase to identify and quantify lymphatic vessels.
  • Detected human beta-globin-related sequences in mouse lymph nodes to confirm human tumor cell presence (metastasis).

Main Results:

  • Tumors with high MLD, specifically PC-14, showed a tendency towards lymph node metastasis.
  • Mice with detectable lymph node metastasis generally exhibited high MLD.
  • Mice without metastasis typically had low MLD, with OST cells being a notable exception.

Conclusions:

  • Microlymphatic vessel density (MLD) is closely associated with lymph node metastasis in this experimental model.
  • High MLD within tumors may serve as a predictive indicator for lymph node metastasis.
  • Further research into MLD's role can refine cancer staging and treatment strategies.