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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Published on: May 13, 2021
Herpes simplex virus type 1-derived recombinant and amplicon vectors.
Cornel Fraefel1, Peggy Marconi, Alberto L Epstein
1Université Lyon 1, Lyon, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2011
Summary
Herpes simplex virus type 1 (HSV-1) vectors offer a promising gene transfer solution due to their broad cell infectivity and high packaging capacity. Researchers have developed efficient methods for preparing recombinant and amplicon vectors derived from HSV-1.
Area of Science:
- Virology
- Gene Therapy
- Molecular Biology
Background:
- Herpes simplex virus type 1 (HSV-1) establishes lytic and latent infections in hosts.
- HSV-1 possesses a large double-stranded DNA genome encoding over 80 genes.
- HSV-1 exhibits unique properties making it suitable for gene transfer applications.
Purpose of the Study:
- To review methods for preparing HSV-1-derived gene transfer vectors.
- To outline strategies for generating recombinant and amplicon vectors.
- To highlight the advantages of HSV-1 as a gene delivery vehicle.
Main Methods:
- Homologous recombination in eukaryotic cells or bacteria for recombinant vectors.
- Bacterial artificial chromosome (BAC)-based approach for amplicon vectors.
- Cre/loxP site-specific recombination for helper-free amplicon vectors.
Main Results:
- Established methodologies for producing recombinant HSV-1 vectors.
- Developed strategies for generating helper-free amplicon HSV-1 vectors.
- Demonstrated the feasibility of using HSV-1 for gene transfer.
Conclusions:
- HSV-1 vectors are valuable tools for gene therapy due to their infectivity, packaging capacity, and non-integrating nature.
- Efficient preparation methods for both recombinant and amplicon vectors are available.
- HSV-1's characteristics minimize risks like insertional mutagenesis in gene transfer applications.
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