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Related Experiment Video

Updated: Jun 1, 2026

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Published on: November 15, 2024

Using restriction mapping to teach basic skills in the molecular biology lab.

Lauren Walsh¹, Elizabeth Shaker, Elizabeth A De Stasio

¹From the Biology Department, Lawrence University, Appleton, Wisconsin 54911.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology

|May 19, 2011

Summary

This lab exercise teaches DNA restriction digestion and gel electrophoresis through hands-on plasmid restriction mapping. Students develop technical skills and learn science writing through data interpretation and figure presentation.

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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

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Last Updated: Jun 1, 2026

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

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Published on: November 15, 2024

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Area of Science:

Molecular Biology
Biotechnology Education

Background:

Restriction enzyme digestion, reagent calculation, and agarose gel electrophoresis are fundamental molecular biology techniques.
Effective teaching of these techniques benefits from repetitive, hands-on laboratory exercises.

Purpose of the Study:

To introduce an investigative laboratory exercise for plasmid restriction mapping.
To enhance students' technical expertise in common molecular biology methods.
To reinforce science writing and figure presentation skills.

Main Methods:

Students perform DNA digestion using restriction enzymes.
Calculations for reagent volumes and concentrations are performed for reactions.
DNA fragments are separated and visualized using agarose gel electrophoresis.

Main Results:

Students gain practical experience in DNA manipulation and analysis.
The exercise provides interpretable data for scientific reporting.
The utility of gel electrophoresis and restriction mapping is explored.

Conclusions:

This open-ended lab exercise effectively integrates technical skill development with scientific interpretation.
It serves as a valuable tool for teaching core molecular biology techniques and scientific communication.