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Related Experiment Video

Updated: Jun 1, 2026

Measuring Lactase Enzymatic Activity in the Teaching Lab

Measuring Lactase Enzymatic Activity in the Teaching Lab

Published on: August 6, 2018

Teaching lactose metabolism: A Complex Challenge Faced with a Simple Kit.

Andrea Monti-Hughes¹, Manuel Alonso, Judith Garófalo

¹From the School of Medicine, Department of Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology

|May 19, 2011

Summary

This study presents a simple, inexpensive classroom experiment for teaching carbohydrate metabolism and lactose intolerance. Hands-on learning with student-discussed results boosted motivation and understanding of these metabolic concepts.

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Area of Science:

Biochemistry
Educational Science

Background:

Carbohydrate metabolism is fundamental to understanding human physiology.
Lactose intolerance is a common metabolic disorder linked to carbohydrate digestion.

Purpose of the Study:

To develop an accessible experimental didactic proposal for teaching carbohydrate metabolism.
To integrate the teaching of lactose intolerance within the context of carbohydrate metabolism.

Main Methods:

Implemented an empirical strategy using inexpensive and nontoxic materials.
Designed experiments requiring no prior laboratory techniques for students.

Main Results:

Students successfully performed experiments in the classroom setting.
Discussion of self-obtained experimental results enhanced student engagement.

Conclusions:

The proposed experimental approach effectively teaches carbohydrate metabolism and lactose intolerance.
Student-led data analysis and discussion significantly increased learning motivation.