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Related Experiment Video

Updated: Jun 1, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Clostridium difficile transcriptome analysis using pig ligated loop model reveals modulation of pathways not

Joy Scaria1, Tavan Janvilisri, Susan Fubini

  • 1Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.

The Journal of Infectious Diseases
|May 20, 2011
PubMed
Summary

This study reveals unique Clostridium difficile pathways active only during infection. These findings highlight potential new therapeutic targets for treating C. difficile infections.

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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

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Related Experiment Videos

Last Updated: Jun 1, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Clostridium difficile is a major cause of healthcare-associated infections.
  • Understanding the in vivo behavior of C. difficile is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the in vivo gene expression profile of Clostridium difficile during infection.
  • To identify novel therapeutic targets by comparing in vitro and in vivo gene expression.

Main Methods:

  • Utilized a pig ligated loop model to simulate C. difficile infection.
  • Performed microarray analysis on bacterial RNA retrieved at various time points.
  • Quantified toxin levels using enzyme-linked immunosorbent assay.

Main Results:

  • Identified differential expression of virulence-associated genes and sporulation cascade genes in vivo.
  • Confirmed upregulation of toxin genes and high toxin production during infection.
  • Discovered differentially expressed genes not present in primary C. difficile annotations.
  • Observed unique pathway expression in vivo compared to in vitro conditions.

Conclusions:

  • Several C. difficile pathways are uniquely modulated during in vivo infection.
  • These infection-specific pathways represent potential targets for new therapeutic interventions against C. difficile.