Related Experiment Video
Updated: Jun 1, 2026
11:49
Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
Published on: September 9, 2011
Developing cell-specific antibodies to endothelial progenitor cells using avian immune phage display technology
Tyrone Bowes1, Shirley A Hanley, Aaron Liew
1Regenerative Medicine Institute, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
Journal of Biomolecular Screening
|May 20, 2011
Summary
Researchers developed chicken phage display libraries to create single-chain antibodies (scFvs) targeting cell surface markers on endothelial progenitor cells (EPCs). This method effectively identifies antibodies against unknown markers on live cells, aiding stem cell research.
Area of Science:
- Immunology
- Biotechnology
- Cell Biology
Background:
- Traditional methods for antibody generation may alter antigen structure.
- Identifying specific cell surface markers on heterogeneous cell populations like endothelial progenitor cells (EPCs) is challenging.
Purpose of the Study:
- To generate immune chicken phage display libraries for single-chain antibodies (scFvs).
- To develop scFvs targeting cell surface markers on live peripheral blood mononuclear cells (PBMCs) containing EPCs.
- To establish a method for screening antibodies against unknown cell surface markers on progenitor/stem cells.
Main Methods:
- Immunization of chickens with live EPCs.
- Phage display library construction and biopanning.
- Purification and characterization of scFvs using flow cytometry.
Main Results:
- A robust immune response was observed after chicken immunization with live EPCs.
- Three unique scFvs (UG1-3) were successfully generated and characterized.
- Recombinant UG1 (gUG-1) demonstrated binding to CD14-positive cells, indicating specificity for monocyte subsets.
Conclusions:
- Chicken immunization combined with phage display is effective for generating scFvs against unknown cell surface markers on heterogeneous cell populations.
- This approach shows significant potential for developing reagents to isolate and characterize stem and progenitor cells.
- The developed scFvs can aid in identifying and isolating specific cell populations.
