Related Experiment Video
Updated: Jun 1, 2026
Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
Published on: February 12, 2014
Automated phase retrieval of a single-material object using a single out-of-focus image
Samuel A Eastwood1, David M Paganin, Amelia C Y Liu
1School of Physics, Monash University, Victoria 3800, Australia. samuel.eastwood@monash.edu
Abstract:
Phase retrieval is widely used in phase contrast microscopy. Here we present an autofocus algorithm that allows the phase of the exit wave function, from a single-material object, to be reconstructed at medium resolution from a single phase contrast image without any a priori knowledge of the imaging system or object. The algorithm is demonstrated on coherent out-of-focus electron micrographs of 30 nm latex sphere calibration standards, giving <10% RMS error over a large defocus range.
