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Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
Tightly bound pyrophosphate in Escherichia coli inorganic pyrophosphatase
A A Shestakov1, A A Baykov, S M Avaeva
1A.N.Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow, USSR.
Abstract:
Hexameric inorganic pyrophosphatase of Escherichia coli contains about 1 mol/mol of 'structural' pyrophosphate, which survives gel filtration and prolonged incubation with Mg2+, does not exchange with medium phosphate and pyrophosphate but is removed with 0.8 M perchloric acid. The site of pyrophosphate binding seems to be another than the active site. An additional 0.9 mol of enzyme-bound pyrophosphate is formed in the presence of phosphate and Mg2+ but this pyrophosphate is in fast equilibrium with medium phosphate and appears to be bound to the active site.
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