Related Experiment Video
Updated: Jun 1, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
No mutations within conserved domain I of the p53 gene in medullary thyroid cancer
C Lang1, M Herrmann, M Elmaghrabi
1SUNY STONY BROOK,DEPT SURG,STONY BROOK,NY 11794. SUNY STONY BROOK,DEPT PATHOL,STONY BROOK,NY 11794. SUNY STONY BROOK,DEPT PHYSIOL & BIOPHYS,STONY BROOK,NY 11794. MICHIGAN CANC FDN,DETROIT,MI 48201.
Abstract:
Abnormal mobility shifts in single strand conformational polymorphism (SSCP)-analysis of exons 2 and 3 of the p53 gene in 2 out of 5 medullary thyroid cancers (MTC) prompted us to sequence the corresponding genomic region spanning codons 9-32. We found no mutations of the conserved domain I in the two MTCs that had previously shown mobility shifts, nor in a third MTC case indicating wild-type p53 (in this region). However, we detected a polymorphism consisting of a GC to CG transversion in intron 2 in two of the primary MTCs and one associated blood sample. In addition, we report on a deviation of the published sequence of intron 2 affecting positions 11817-8 and 11874, seen in 7/7 samples analyzed. Our findings corroborate the hypothesis, that MTC carcinogenesis does not require p53 mutations (Yana et al, Jpn J Cancer Res 1992).
More Related Videos
Related Concept Videos
Abnormal Proliferation
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

