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Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
Tissue factor mRNA expression in breast carcinoma cells.
J Zhou1, M Stenlinder, S Linder
1KAROLINSKA HOSP & INST,RADIUMHEMMET,DEPT GEN ONCOL,RES LAB,S-17176 STOCKHOLM,SWEDEN. KAROLINSKA HOSP & INST,DEPT CLIN CHEM,S-17176 STOCKHOLM,SWEDEN.
Oncology Reports
|May 20, 2011
Summary
Tissue factor (TF) mRNA expression is not exclusive to malignant breast cells. TF mRNA was detected in both cancerous and non-cancerous breast cell lines and in node-negative breast cancers.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Tissue factor (TF) initiates the extrinsic coagulation pathway and has been detected in tumor cells.
- Previous observations indicated high TF mRNA expression in metastatic breast cancer cell lines.
Purpose of the Study:
- To investigate TF mRNA expression in various breast cell lines and primary tumors.
- To determine if TF expression correlates with the malignant potential of breast cells.
Main Methods:
- Cloning of TF cDNA based on differential mRNA expression in MDA-MB-231 and MCF-7 cells.
- Analysis of TF mRNA levels in immortalized non-malignant and malignant breast epithelial cell lines.
- RT-PCR analysis of TF mRNA in node-negative breast carcinoma tissues.
Main Results:
- TF mRNA was highly expressed in nonmalignant immortalized breast epithelial cell lines (A1N4, MCF-10, HBL100).
- Low TF mRNA levels were observed in four breast carcinoma-derived cell lines.
- Strong TF mRNA expression was detected in 10 out of 24 node-negative breast carcinoma samples.
Conclusions:
- TF expression levels do not correlate with the malignant potential of the examined breast cell lines.
- TF mRNA is detectable in node-negative breast cancers, suggesting a role beyond metastasis initiation.
