Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Karyotyping

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...

Nondisjunction

Nondisjunction

During meiosis, chromosomes occasionally separate improperly. This occurs due to failure of homologous chromosome separation during meiosis I or failed sister chromatid separation during meiosis II. In some species, notably plants, nondisjunction can result in an organism with an entire additional set of chromosomes, which is called polyploidy. In humans, nondisjunction can occur during male or female gametogenesis and the resulting gametes possess one too many or one too few chromosomes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clonal KEAP1 mutations with loss of heterozygosity share reduced immunotherapy efficacy and low immune cell infiltration in lung adenocarcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2022

Same author

Guiding antiferromagnetic transitions in Ca[Formula: see text]RuO[Formula: see text].

Scientific reports·2022

Same author

Unveiling unconventional magnetism at the surface of Sr<sub>2</sub>RuO<sub>4</sub>.

Nature communications·2021

Same author

A Calibrated Multiexit Neural Network for Detecting Urothelial Cancer Cells.

Computational and mathematical methods in medicine·2021

Same author

Prognostic role of immunohistochemical overexpression of the p16 protein in women under the age of 35 and diagnosed with HSIL (CIN2) subjected to "cervix sparing" excision.

European review for medical and pharmacological sciences·2021

Same author

KEAP1-driven co-mutations in lung adenocarcinoma unresponsive to immunotherapy despite high tumor mutational burden.

Annals of oncology : official journal of the European Society for Medical Oncology·2020

Same journal

Identification of RRM2 as a key regulator of malignant epithelial cells in gastric cancer through single‑cell transcriptomics.

Oncology reports·2026

Same journal

Impact of WFDC2 on the biological functions of cervical cancer cells and the regulation of the ERK/NF‑κB signaling pathway.

Oncology reports·2026

Same journal

Schisandrin B exhibits anti‑proliferative effects by inducing ferroptosis in pancreatic cancer.

Oncology reports·2026

Same journal

Research advances on DKK1 in head and neck squamous cell carcinoma: Linking molecular mechanisms to clinical applications (Review).

Oncology reports·2026

Same journal

PI3Kδ inhibitor YY‑20394 is effective alone or in combination with Bcl‑2 inhibitor ABT199 in acute myeloid leukemia cells.

Oncology reports·2026

Same journal

A fibroblast activation protein degrader enhances cisplatin sensitivity in non‑small cell lung cancer.

Oncology reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Aneuploid polyclonality in image analysis.

M Alderisio¹, G Ribotta, E Giarnieri

¹UNIV ROMA LA SAPIENZA,CLIN CHIRURG 6,ROME,ITALY. UNIV ROMA LA SAPIENZA,CATTEDRA STAT MED & BIOMETRIA,DIPARTIMENTO MED SPERIMENTALE & PATOL,ROME,ITALY.

Oncology Reports

|May 20, 2011

Summary

This summary is machine-generated.

Colorectal cancer cell DNA ploidy analysis reveals that aneuploid and polyclonal cases are linked to increased lymph node infiltration and poorer disease-free survival, indicating a higher relapse risk.

More Related Videos

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

Published on: February 15, 2022

Related Experiment Videos

Last Updated: Jun 1, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence

Published on: January 27, 2023

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

Published on: February 15, 2022

Area of Science:

Oncology
Genetics
Pathology

Background:

Colorectal adenocarcinomas exhibit diverse cell subpopulations.
Intranuclear DNA quantification techniques assess tumor cell DNA content.
DNA ploidy analysis is a method for studying cellular DNA content.

Purpose of the Study:

To evaluate the prognostic significance of polyclonal colorectal carcinoma cases.
To correlate DNA ploidy with lymph node infiltration and disease-free survival.
To compare pathological staging with DNA ploidy findings.

Main Methods:

Analysis of DNA ploidy in 68 colorectal carcinoma patients using a CAS 200 image analyzer.
Evaluation of neoplastic tissue and healthy mucosa from surgical resections.
Assessment of approximately 150-300 cells per sample.

Main Results:

Aneuploidy and multiple stemlines were significantly associated with lymphatic metastasis (Stage III).
Polyclonality correlated with distant metastasis (Stage IV).
Aneuploid and polyclonal cases showed significantly reduced disease-free survival, with polyclonal cases having a ninefold greater relapse risk.

Conclusions:

DNA ploidy, specifically aneuploidy and polyclonality, serves as a significant prognostic marker in colorectal carcinoma.
These findings highlight the association between abnormal DNA content and advanced disease stages, metastasis, and patient outcomes.
Understanding DNA ploidy patterns can aid in predicting relapse risk and informing treatment strategies.