Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Racket Sports-Related Injuries in Youth Athletes: A Narrative Review.

International journal of environmental research and public health·2026
Same author

Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes.

Human genetics·2026
Same author

Molecular phenotypes in early gallbladder and liver disease in the <i>CFTR</i><sup>-/-</sup> sheep highlight aspects of cystic fibrosis-relevant hepatobiliary disease.

Physiological genomics·2025
Same author

CFTR Gene Regulation in Human Pancreatic Duct, Bile Duct and Sweat Gland Epithelial Cells.

Journal of cellular and molecular medicine·2025
Same author

The Mental Health of Children with Cerebral Palsy: A Review of the Last Five Years of Research.

Journal of clinical medicine·2025
Same author

Cellular imbalance in proximal and distal lung of CFTR<sup>-/-</sup> sheep in utero and at birth.

Molecular medicine (Cambridge, Mass.)·2025

Related Experiment Video

Updated: Jan 1, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.5K

Introduction to Section II: RNA methods to approach CFTR expression.

Ann Harris1

  • 1Human Molecular Genetics Program, Children's Memorial Research Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60614, USA. ann-harris@northwestern.edu

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2011
PubMed
Summary

This review covers methods for analyzing the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) gene and its mRNA. It details techniques for measuring CFTR transcripts, assessing decay pathways, and exploring gene regulation in airway diseases.

More Related Videos

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
15:12

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

Published on: May 10, 2014

14.9K
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
14:56

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

Published on: March 10, 2012

19.0K

Related Experiment Videos

Last Updated: Jan 1, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.5K
Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
15:12

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae

Published on: May 10, 2014

14.9K
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
14:56

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

Published on: March 10, 2012

19.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Expression Analysis

Background:

  • This review focuses on analytical methodologies for the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) gene and its transcript.
  • It addresses the measurement of CFTR mRNA in human cells and transcripts with premature termination codons.

Discussion:

  • The review details protocols for evaluating nonsense-mediated decay impacting CFTR transcripts.
  • Methodologies for investigating pre-mRNA splicing of CFTR are discussed.
  • The role of microRNA in regulating CFTR gene expression within airway diseases is examined.

Key Insights:

  • Advanced techniques for assessing CFTR transcript levels and processing are presented.
  • Methods for understanding the regulatory mechanisms of CFTR, including microRNA and chromatin structure, are outlined.
  • This work provides a comprehensive overview of CFTR analysis relevant to airway disease research.

Outlook:

  • Future research can leverage these methods to develop targeted therapies for CFTR-related disorders.
  • Further investigation into cis-acting regulatory elements controlling CFTR locus activity is warranted.
  • Continued refinement of gene expression analysis techniques will enhance our understanding of CFTR function and dysfunction.