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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Related Experiment Video

Updated: Jun 1, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Approaches to study CFTR pre-mRNA splicing defects.

Elisa Goina1, Eugenio Fernandez-Alanis, Franco Pagani

  • 1Human Molecular Genetics, International Centre for Genetic Engineering and Biotechnology, 34149, Trieste, Italy. egoina@sissa.it

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2011
PubMed
Summary

Genomic variants in cystic fibrosis (CF) can disrupt CFTR mRNA processing. This study explores hybrid minigene tools to assess the pathological impact of CFTR DNA variations on pre-mRNA splicing.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: Jun 1, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Cystic Fibrosis (CF) is linked to genomic variants affecting CFTR precursor mRNA processing.
  • Splicing complexity in CFTR gene necessitates evaluation of variant pathogenicity.
  • Splicing regulatory elements, often overlapping coding sequences, are crucial for CFTR gene function.

Purpose of the Study:

  • To investigate the pathological effects of CFTR DNA variations on pre-mRNA splicing.
  • To describe tools for evaluating splicing alterations in the context of CF.
  • To enhance understanding of basic regulatory mechanisms in gene splicing.

Main Methods:

  • Utilizing hybrid minigene-based tools to assess splicing.
  • Analyzing the impact of CFTR DNA variations on pre-mRNA splicing.
  • Identifying splicing regulatory elements within CFTR transcripts.

Main Results:

  • The described tools enable evaluation of CFTR DNA variations' effect on splicing.
  • Methodologies facilitate determination of the pathological impact of splicing-affecting variants.
  • Insights into the complex splicing mechanisms of the CFTR gene are provided.

Conclusions:

  • Hybrid minigene tools are effective for studying CFTR splicing defects.
  • Accurate assessment of splicing variants is vital for CF diagnosis and research.
  • Understanding splicing regulation is key to deciphering CFTR gene function.