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[A new optical switch using upconversion nanoparticles conjugated aptamer].

Kai Song1, Xiang-gui Kong

  • 1Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China. 22533734@163.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|May 21, 2011
PubMed
Summary
This summary is machine-generated.

This study presents novel upconversion nanoparticles (UCNPs) as fluorescent biolabels for an optical switch. The UCNPs-aptamer system detects thrombin protein by changes in fluorescence intensity.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Materials Science

Context:

  • Traditional fluorescent labels suffer from autofluorescence and scattered excitation light.
  • Upconversion nanoparticles (UCNPs) offer an alternative with near-infrared (NIR) excitation, overcoming these limitations.
  • Water-soluble NaYF4:Yb3+, Er+ UCNPs were synthesized using a solvothermal method with branched polyethylenimine surfactants.

Purpose:

  • To develop a generic design strategy for UCNP-conjugated aptamer-based optical switches.
  • To create a sensitive detection system for specific biomolecules, exemplified by thrombin protein.

Summary:

  • A novel optical switch was designed using UCNPs conjugated with an aptamer.
  • In the absence of thrombin, UCNPs and TAMRA are in proximity, causing luminescence resonance energy transfer (LRET).
  • Upon thrombin introduction, the aptamer forms a quadruplex, displacing TAMRA and increasing UCNP fluorescence, signaling detection.

Impact:

  • This UCNP-aptamer optical switch offers a sensitive and specific method for biomolecular detection.
  • The strategy provides a versatile platform for developing various biosensors.
  • Eliminates autofluorescence and scattered light issues common in traditional fluorescent assays.