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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
13:58

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Published on: June 25, 2014

Simulation of cutaneous Bowen's disease by freeze artifact in tissue briefly fixed in formalin

Hobart W Walling, Brian L Swick

    International Journal of Dermatology
    |May 21, 2011
    PubMed
    Abstract

    No abstract available in PubMed .

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    Fixation and Sectioning01:03

    Fixation and Sectioning

    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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