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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
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Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

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Relationship between angiotensin II receptor expression and cardiovascular risk factors in Mexican patients with

Manuel Baños1, Monica G Arellano-Mendoza, Hilda Vargas-Robles

  • 1Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional México City, C.P. 07360, Mexico.

Experimental and Molecular Pathology
|May 21, 2011
PubMed

Insights

Cardiovascular risk factors and the ACE I/D DD genotype increase Angiotensin II AT1 receptor expression in the aorta. This heightened expression is linked to coronary artery disease development.

Area of Science:

  • Cardiovascular Medicine
  • Molecular Biology
  • Genetics

Background:

  • Angiotensin II (Ang) receptor density is influenced by various factors and pathological conditions.
  • The role of cardiovascular risk factors in regulating Angiotensin II receptor expression is not fully understood.

Purpose of the Study:

  • To investigate the association between cardiovascular risk factors and Angiotensin II AT1 and AT2 receptor expression in human aortic arteries.
  • To explore the link between ACE I/D genotype and Angiotensin II receptor expression.

Main Methods:

  • Ex-vivo study using human aortic postsurgical specimens from 31 Mexican patients with coronary artery disease.
  • Immunostaining to evaluate Angiotensin II receptor expression.
  • Polymerase chain reaction to determine angiotensin converting enzyme insertion/deletion (ACE I/D) polymorphisms.

Main Results:

  • Both AT1 and AT2 receptor expression were elevated in aortic segments of cardiovascular patients compared to controls.
  • Patients with the DD genotype showed increased AT1 and AT2 receptor expression.
  • Increased AT1 receptor expression correlated with the number of cardiovascular risk factors and decreased with drug combinations.
  • No such correlations were observed for AT2 receptor expression.

Conclusions:

  • Increased AT1 receptor expression is associated with the DD genotype and the presence of cardiovascular risk factors.
  • Elevated AT1 receptor expression may contribute to the development of coronary occlusive disease.

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