Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimal Stenting Strategy During Chemotherapy: Impact of Time to First Reintervention on Survival in Malignant Hilar Biliary Obstruction.

DEN open·2026
Same author

Endoscopic retrieval of an N-butyl-2-cyanoacrylate cast from the common bile duct: a rare complication of portal vein embolization.

Endoscopy·2026
Same author

Activation of the Integrin αV-YAP-CTGF Axis in Liver Sinusoidal Endothelial Cells Promotes Liver Fibrogenesis, Leading to Portal Hypertension and Liver Carcinogenesis in Congestive Hepatopathy.

Gastroenterology·2026
Same author

Geometrical preference of anchoring sites in the unicellular organism <i>Stentor coeruleus</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Full-Coverage Drug-Eluting Stent Vs Drug-Coated Balloon in De Novo Femoropopliteal Lesions: Three-Year TASC II-Stratified Outcomes.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2026
Same author

Preliminary analysis for the ADD-BP study: Atypical femoral fractures and physical activity levels in bisphosphonate users.

Fukushima journal of medical science·2026

Related Experiment Video

Updated: Jun 1, 2026

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Avidin/PSS membrane microcapsules with biotin-binding activity.

Yoshihiro Endo1, Katsuhiko Sato, Kentaro Sugimoto

  • 1Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.

Journal of Colloid and Interface Science
|May 21, 2011
PubMed
Summary
This summary is machine-generated.

Avidin-poly(styrene sulfonate) polyelectrolyte microcapsules were created for enhanced uptake and release of biotin-labeled molecules. These microcapsules successfully immobilized biotin-labeled glucose oxidase, retaining its activity.

More Related Videos

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

Related Experiment Videos

Last Updated: Jun 1, 2026

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
10:43

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Layer-by-layer deposition is a versatile technique for creating functional microstructures.
  • Avidin-biotin interactions are highly specific and widely used in bioconjugation.
  • Polyelectrolyte microcapsules offer controlled environments for molecular encapsulation and release.

Purpose of the Study:

  • To prepare polyelectrolyte microcapsules with avidin-poly(styrene sulfonate) (PSS) membranes.
  • To investigate the uptake and release dynamics of biotin-labeled fluorescein (b-FITC).
  • To study the immobilization of biotin-labeled glucose oxidase (b-GOx) onto the microcapsules.

Main Methods:

  • Layer-by-layer deposition of avidin/PSS on calcium carbonate (CaCO(3)) microparticles.
  • Dissolution of CaCO(3) core using ethylenediaminetetraacetic acid (EDTA).
  • Characterization of microcapsule formation and functionalization with b-FITC and b-GOx.

Main Results:

  • Avidin/PSS microcapsules demonstrated significantly enhanced uptake of b-FITC due to specific avidin-biotin binding.
  • Release of b-FITC was triggered and accelerated by the addition of free biotin, indicating competitive binding.
  • Biotin-labeled glucose oxidase (b-GOx) was successfully immobilized on the microcapsule surface while maintaining its catalytic activity.

Conclusions:

  • Avidin-functionalized polyelectrolyte microcapsules provide a robust platform for controlled capture and release of biotinylated molecules.
  • The developed microcapsules are suitable for immobilizing enzymes like glucose oxidase, preserving their biological function.
  • This approach holds potential for applications in biosensing, drug delivery, and bioseparation.