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Related Experiment Video

Updated: Jun 1, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

Segmental versican expression in the trabecular meshwork and involvement in outflow facility.

Kate E Keller¹, John M Bradley, Janice A Vranka

¹Casey Eye Institute, Oregon Health and Science University, Portland, Oregon 97239, USA.

Investigative Ophthalmology & Visual Science

|May 21, 2011

Summary

Versican, a key proteoglycan in the trabecular meshwork (TM), influences aqueous humor outflow. Its silencing altered outflow facility differently in human and porcine eyes, suggesting species-specific roles in regulating eye pressure.

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Area of Science:

Ocular Biology
Biochemistry
Proteoglycan Research

Background:

Versican is a large proteoglycan with chondroitin sulfate (CS) glycosaminoglycan (GAG) side chains.
Its role in aqueous humor outflow resistance is not fully understood.

Purpose of the Study:

To investigate versican's distribution in the trabecular meshwork (TM).
To determine versican's effect on aqueous humor outflow facility.

Main Methods:

Used fluorescent quantum dots (Qdots) to label outflow pathways.
Employed immunofluorescence and RT-PCR for versican distribution analysis.
Utilized lentiviral shRNA to silence versican and CS GAG synthesis in anterior segment cultures.

Main Results:

Versican levels varied segmentally within the TM, inversely correlating with Qdot labeling.
Silencing versican decreased outflow facility in human eyes but increased it in porcine eyes.
Silencing CS GAG synthesis increased outflow facility in both species.

Conclusions:

Versican is integral to outflow resistance, potentially organizing ECM components for flow.
The molecular mechanisms of outflow resistance involving versican differ between human and porcine eyes.