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Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for
Daniel C Propheter1, Lara K Mahal
1Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX 78712-0265, USA.
Molecular Biosystems
|May 21, 2011
Abstract:
Herein we describe the orientation of GST-tagged lectins on NHS-activated slides via a one-step deposition of the protein and a glutathione (GSH) scaffold. This technology overcomes the need for a premade GSH-surface to orient GST-tagged proteins, enabling us to rapidly expand the analytical capacity of lectin microarrays through addition of oriented lectins, while maintaining lectin diversity.
