Related Experiment Video
Updated: Jun 1, 2026
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Published on: May 9, 2020
Chemosensitivity of gastric-cancer using adhesive tumor-cell culture system
M Kitamura1, H Furukawa, T Kito
1DEPT SURG ADULT DIS CANC,OSAKA,JAPAN. AICHI CANC CTR,DEPT SURG,NAGOYA,AICHI 464,JAPAN. NIIGATA CANC CTR,DEPT SURG,NIIGATA,JAPAN. AICHI CANC CTR,DEPT INTERNAL MED,NAGOYA,AICHI 464,JAPAN. SRL INC,TOKYO,JAPAN. NATL SENDAI HOSP,DEPT SURG,SENDAI,JAPAN. CANC INST HOSP,DEPT SURG,TOKYO,JAPAN. TOKYO METROPOLITAN KOMAGOME HOSP,JAPANESE GASTR CANC SURG STUDY GRP,BUNKYO KU,TOKYO 113,JAPAN.
Abstract:
A total of 91 specimens of surgically resected tumors from 80 patients with gastric cancer were assayed for chemosensitivity using an adhesive tumor cell culture system (Life Trac ATCCS assay). Seventy-eight specimens of 91 had sufficient number of cells to grow in culture and 64 (82%) were evaluable excluding 8 low growth and 6 fungus contaminations. Cells (3x10(3)/ml/well) were cultured for 14 days and exposed to drugs on days 3-8. The growing cells were confirmed as cancer cells by immunohistochemical staining using monoclonal antibody to cytokeratin, epithelial membrane antigen and vimentin. IC90 value against (ADM, CDDP, CPM, 5-FU, MMC, MTX, VP-16, CBDCA and MMC+5-FU+MTX) was determined and population distribution of IC90 for each drug was obtained to serve as basic data for judging sensitivity. The 10th percentile of IC90 (mug/ml) was 0.01, 0.43, 1.23, 0.23, 0.01, 0.005, 0.14, 1.56 and 0.009+0.05+0.003 and median of IC90 was 0.02, 0.99, 2.31, 0.30, 0.06, 0.01, 0.39, 3.19 and 0.02+0.10+0.005, respectively. Population distribution of IC90 against each drug showed a specific pattern. Profiles of IC90 against various anticancer drugs differed in individual patients. Chemosensitivity of lymph node metatases seemed to be more resistant than that of their primary tumors. The ATTCS test was found to be useful as a sensitivity test for anti-cancer agents because of its reliability and excellent quantification.