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Increased expression and phosphorylation of sh-ptp2 (syp) in human gastric carcinomas

K Kitahara1, W Yasui, H Kuniyasu

  • 1HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,MINAMI KU,HIROSHIMA 734,JAPAN. SAGA MED SCH,DEPT SURG,SAGA 849,JAPAN.

Oncology Reports
|May 21, 2011
PubMed

Insights

Increased expression and tyrosine-phosphorylation of SH-PTP2 (SYP) were observed in stomach cancer tissues and cell lines. These findings suggest SH-PTP2 may play a role in stomach carcinogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • SH-PTP2 (SYP) is a novel protein tyrosine phosphatase.
  • Its role in gastric carcinoma is not well understood.

Purpose of the Study:

  • To investigate the expression and phosphorylation status of SH-PTP2 in human gastric carcinoma.
  • To determine the potential involvement of SH-PTP2 in stomach carcinogenesis.

Main Methods:

  • Northern blotting to detect SH-PTP2 mRNA levels.
  • Western blotting to assess SH-PTP2 protein expression and phosphorylation.
  • Analysis of gastric carcinoma cell lines and tissues compared to non-neoplastic mucosas.

Main Results:

  • SH-PTP2 mRNA was detected in all 8 gastric carcinoma cell lines.
  • 14 out of 21 gastric carcinomas (66.6%) showed higher SH-PTP2 mRNA levels than non-neoplastic mucosas.
  • Gastric carcinomas predominantly displayed tyrosine-phosphorylated SH-PTP2, while non-neoplastic tissues showed unphosphorylated forms.

Conclusions:

  • Elevated expression and tyrosine-phosphorylation of SH-PTP2 are associated with human gastric carcinoma.
  • These alterations suggest SH-PTP2 may contribute to the development of stomach cancer.

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