Related Experiment Video

Updated: Jun 1, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
09:20

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

Human papillomavirus type-16 DNA integration in nuclear matrix and chromosome scaffold associated DNA in a

Y Yam1, E Chew, L Yang

  • 1CHINESE UNIV HONG KONG,DEPT ANAT,SHA TIN,HONG KONG. CHINESE UNIV HONG KONG,DEPT PHYSIOL,SHA TIN,HONG KONG. BEIJING UNIV,DEPT CELL BIOL,BEIJING,PEOPLES R CHINA.

Oncology Reports
|May 21, 2011
PubMed

Insights

Matrix attachment regions (MARs) and scaffold attachment regions (SARs) bind human papillomavirus type 16 DNA in cervical cancer cells. This association suggests MARs/SARs may enhance viral transcription in transformed cells.

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • Matrix attachment regions (MARs) and scaffold attachment regions (SARs) are DNA elements that anchor chromatin to the nuclear matrix and scaffold.
  • These regions are implicated in DNA replication and transcription regulation.
  • Human papillomavirus (HPV) type 16 is a key etiological agent in cervical carcinoma.

Purpose of the Study:

  • To investigate the association of HPV type 16 DNA with MARs and SARs.
  • To explore the potential role of this association in viral gene expression within cervical cancer cells.

Main Methods:

  • Polymerase chain reaction (PCR) was employed to detect HPV DNA.
  • The study utilized the CC3/CUHK3 cervical carcinoma cell line.

Main Results:

  • The study demonstrated the presence of HPV type 16 DNA associated with MARs and SARs in the CC3/CUHK3 cell line.
  • This physical association was confirmed through molecular techniques.

Conclusions:

  • The findings provide evidence for the interaction between HPV type 16 DNA and host cell nuclear matrix/scaffold attachment regions.
  • This association may contribute to the elevated transcription rates of the viral genome observed in transformed cervical cells.

More Related Videos

Merkel Cell Polyomavirus Infection and Detection13:45

Merkel Cell Polyomavirus Infection and Detection

Published on: February 7, 2019

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

Related Concept Videos

Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...