Perfusion method for bone marrow cell collection in poor mobilizer lymphoma patient
Shinichiro Mori1, Shinya Fujita1, Yoshihisa Yamamoto1
1The First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan.
Abstract:
We previously described a unique procedure for the collection of bone marrow cells (BMCs) using a perfusion method (PM). In cynomolgus monkeys, this method resulted in lower contamination with T cells (<10%). Here, we performed PM on a poor mobilizer lymphoma patient. We confirmed the safety of the intra-bone marrow injection of saline to collect the BMCs. The collected BMCs showed minimal contamination with T cells (<15%) and red blood cells (RBCs) (<4%) from the peripheral blood. It took a total of only 30 min to collect the BMCs. Moreover, transfusion of RBCs was unnecessary. There were no relevant post-operative side effects except for self-limiting pain at the sites of collection, and the patient was able to walk around the hospital after the operation.
Insights
The novel perfusion method (PM) safely collects bone marrow cells (BMCs) with minimal T cell and red blood cell (RBC) contamination in lymphoma patients. This rapid, 30-minute procedure eliminates the need for RBC transfusions and allows patients to ambulate post-operation.
Area of Science:
- Hematology
- Cellular Therapy
- Surgical Procedures
Background:
- Traditional bone marrow collection methods can lead to significant T cell contamination.
- Poor mobilizers pose challenges for autologous stem cell transplantation.
- The perfusion method (PM) was previously validated in non-human primates.
Observation:
- The safety of intra-bone marrow saline injection for BMC collection was confirmed.
- The perfusion method (PM) was applied to a lymphoma patient who was a poor mobilizer.
- Patient recovery was rapid, with minimal side effects.
Findings:
- Perfusion method (PM) yielded bone marrow cells (BMCs) with low T cell (<15%) and red blood cell (RBC) (<4%) contamination.
- The procedure was efficient, taking only 30 minutes for BMC collection.
- No red blood cell (RBC) transfusion was required post-collection.
Implications:
- The perfusion method (PM) offers a promising alternative for bone marrow cell (BMC) collection in challenging patient populations.
- This technique may improve the quality of collected cells for transplantation.
- The minimally invasive nature and speed of PM enhance patient comfort and reduce recovery time.
