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[Application of quantum dots fluorescent probes in tissue of human tongue squamous cell carcinoma]
Jian-Jiang Zhao1, Yu-Hua Huang, Zhi-Ping Wang
1Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southern Medical University; Guangdong Provincial Stomatological Hospital, Guangzhou 510280, China.
Objective:
To study if quantum dots fluorescent probes can be applied to detect P53 protein and Bcl-2 protein in tissue of human tongue squamous cell carcinoma.
Methods:
By indirect immunofluorescence assay the same particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein respectively. Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope.
Results:
P53 protein and Bcl-2 protein can be combined with quantum dots fluorescent probes and specific fluorescene can be observed with ultraviolet light excited. P53 protein was mainly distributed in the nucleus, and Bcl-2 protein major in the cytoplasm. P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carcinoma and two kinds of fluorescene can be observed.
Conclusion:
Quantum dots fluorescent probes can be applied to detect two kinds of specific protein in paraffin-embedded tissue section of human tongue squamous cell carcinoma.
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