Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Principles Of Column Chromatography

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

22q11.2 duplication syndrome and LZTR1-related Noonan syndrome type 10 overlapping phenotypes in a dual diagnosis - fetal hydrops: a case report.

BMC pregnancy and childbirth·2026

Same author

Personalizing post-stroke symptom management: integrating network analysis and in silico intervention to identify subgroup-specific targets.

BMC neurology·2026

Same author

Exercise modulates food reward: neurobiological mechanisms and implications for weight management.

Frontiers in nutrition·2026

Same author

Letter to the Editor in response to the article "Association between thyroid dysfunction and cognitive impairment in type 2 diabetes mellitus: A cross-sectional study".

Journal of diabetes investigation·2026

Same author

Single-Cell Proteomics Decodes the Cellular Response to Lysosomal Storage in <i>C. elegans</i> Coelomocytes.

International journal of molecular sciences·2026

Same author

Simultaneous In-Depth Single-Cell Proteomic and Metabolomic Analysis.

Analytical chemistry·2026

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

[Developments and applications of microfluidic chip-based chromatographic technique].

Xinjue Wang¹, Ying Zhu, Qun Fang

¹Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.

Se Pu = Chinese Journal of Chromatography

|May 24, 2011

Summary

Microfluidic chip-based chromatography is advancing rapidly. This review covers progress in fabrication, component design, system integration, and applications of these miniature chromatographic systems.

More Related Videos

A Microfluidic Chip for ICPMS Sample Introduction

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Jun 1, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Microfluidic Chip for ICPMS Sample Introduction

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

Analytical Chemistry
Separation Science
Microfluidics

Context:

Microfluidic chip-based chromatography integrates separation processes onto miniaturized platforms.
Recent advancements have significantly improved the performance and applicability of these systems.
The field is characterized by rapid innovation in component design and integration.

Purpose:

To review recent progress in microfluidic chip-based chromatographic techniques.
To highlight advancements in the fabrication of chromatographic columns.
To discuss the design of integrated valves and pumps for microfluidic systems.

Summary:

Recent progress in microfluidic chip-based chromatography includes enhanced column fabrication and sophisticated valve/pump designs.
Integration of these components into single chips facilitates complex separations.
Diverse applications showcase the growing utility of these miniaturized chromatographic systems.

Impact:

Accelerated development of portable and high-throughput analytical devices.
Enabled novel applications in fields requiring sensitive and rapid chemical analysis.
Provided a comprehensive overview of the state-of-the-art for researchers and developers.